B3GNT2 Knockout cell line (A-375)

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B3GNT2 Knockout cell line (A-375) Order

B3GNT2 Knockout cell line (A-375)
Catalog#
YKO-H486
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
B3GNT2 Knockout cell line (A-375)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
B3GNT2 Knockout cell line (A375)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
B3GNT2
Gene ID
10678
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D7J4(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript B3GNT2-201)
Validation: PCR+Sanger Sequencing
B3GNT2 Knockout cell line (A-375) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A-375		 STR Info (Cell bank)
Cell Line:A-375 [A375]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 9999
D16S539 9999
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 810810
CSF1PO 11121112
D12S391 1821
FGA 2323
D2S1338 1624
D21S11 2930
D18S51 1217
D8S1179 1114
D3S1358 1517
D6S1043 1114
PENTAE 1012
D19S433 1314.2
PENTAD 915
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the B3GNT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A-375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, B3GNT2 gene knockout B3GNT2 Knockout cell line (A375) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
B3GNT2 Knockout cell line (A-375)
B3GNT2 Knockout cell line (A-375)
Catalog#
YKO-H486
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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